Characterisation of gold electrodes modified with self-assembled monolayers of l-cysteine for the adsorptive stripping analysis of copper

Electrochemical sensors for copper ions in environmental samples were prepared by modifying gold electrodes with l-cysteine by self-assembly. The adsorption of l-cysteine on gold electrodes was studied by electrochemical reductive desorption in 0.5 M KOH, and the interaction of l-cysteine with copper ions was investigated by cyclic voltammetry, chronoamperometry and X-ray photoelectron spectroscopy. At low concentrations the ratio of l-cysteine to bound Cu(II) is 2:1. At higher concentrations (0.1 M) copper reacts with adsorbed cysteine forming copper sulfide on the electrode surface. On a modified l-cysteine gold electrode, Osteryoung square wave voltammetric determination of Cu(II) with a detection limit below 5 ppb has been demonstrated.

Simple and signal-off electrochemical biosensor for mercury(II) based on thymine-mercury-thymine hybridization directly on graphene

Abstract A simple, signal-off and reusable electrochemical biosensor was developed for sensitive and selective detection of mercury(II) based on thymine-mercury(II)-thymine (T-Hg²⁺-T) complex and the remarkable difference in the affinity of graphene with double strand DNA (ds-DNA) and single strand DNA (ss-DNA). Our system was composed of ferrocene-tagged probe DNA and graphene. Due to the noncovalent assembly, the ferrocene-tagged probe ss-DNA was immobilized on the surface of graphene nanosheets directly and employed to amplify the electrochemical signal. In the presence of Hg²⁺, the ferrocene-labeled T-rich DNA probe hybridized with target probe to form ds-DNA via the Hg²⁺-mediated coordination of T-Hg²⁺-T base pairs. As a result, the duplex DNA complex kept away from the graphene surface due to the weak affinity of graphene and ds-DNA, and the redox current decreased substantially. Meanwhile, the graphene decorated GCE surface was released for the reusability. Under the optimal conditions, the proposed sensor showed a linear concentration range from 25 pM to 10 μM with a detection limit of 5 pM for Hg²⁺ detection. The strategy afforded exquisite selectivity for Hg²⁺ against other metal ions in real environmental samples.

Physical environmental conditions, spawning and early-life stages of an estuarine fish : climate change implications for recruitment in intermittently open estuaries

Significant variation in the egg and larval survival and juvenile recruitment of estuarine fishes has been linked to fluctuating environmental conditions. This present study compared the distribution and abundance of black bream (Acanthopagrus butcheri) eggs and yolk-sac larvae between two microtidal estuaries of different flow regimes, where the riverine flow into the Glenelg estuary was around eight times the flow volume into the Hopkins estuary. Samples were collected monthly from September to November at sites along each estuary where vertical profiles of temperature, salinity and dissolved oxygen (DO) were measured, and vertically stratified sampling of black bream eggs and yolk-sac larvae was conducted using a Schindler sampler. Salt wedge formation was apparent in both estuaries, with significant de-oxygenation of deeper, saline waters. Eggs occurred in a wide range of DO levels but yolk-sac larvae were less common at the lowest levels. Most eggs and yolk-sac larvae were collected in salinities greater than 10. Results suggested that egg mortality was higher in the Hopkins than the Glenelg estuary, which may be associated with the hypoxic conditions characteristic of low-flow conditions. The results have significant implications in terms of climate change that is predicted to lead to warmer, drier conditions in south-eastern Australia, potentially increasing stratification and subsequent hypoxic zones.

Environmental impact assessment of the sewage outfall at Davis Station, East Antarctica

During the summer 2009/10, an environmental impact assessment of the sewage outfall was conducted at Davis Station, East Antarctica. An investigation of the nature and extent of impacts to the marine environment associated with current sewage treatment and effluent discharge practices included ecological surveys of macrobiological communities, physico-chemical analysis of sediments and receiving waters, microbiological analysis, and histopathological analysis of fish. Ecotoxicological testing using local invertebrates to determine effluent toxicity was interpreted alongside dispersal modelling data of the discharge plume to determine the potential extent of impacts and inform recommendations on the level of treatment and dilution of sewage required to minimise impacts. No evidence of impacts was detected on soft sediment infaunal or epifaunal communities, and only low levels of contamination and accumulation were found in sediments and waters in the immediate vicinity of the outfall and in the direction of primary current flow. In contrast, sterol biomarkers and faecal coliforms (E. coli) were detected in sediments collected adjacent to the outfall and in most water column samples. Marine invertebrates (Abatus and Laternula) also tested positive for E. coli and antibiotic resistance mechanisms were present in Laternula indicating the introduction and dispersal through the water column of foreign microbes and bacteria associated with human effluent. Fish (Trematomus bernacchii) close to the outfall showed significant histological alterations in all major tissues (liver, gill, gonad, muscle) resulting from exposure to sewage. Effluent was toxic to amphipods (Paramoera walkeri) and microgastropods (Skenella paludionoides), with reduced survival in concentrations as low as 3.125% over a 21d exposure period. Acute effects were also observed in both species following 24h exposure, with 100% mortality at the highest effluent concentrations tested (68%). The application of these results to support and guide decisions regarding the planned installation of new sewage treatment facilities at Davis will be discussed.

A study of the impact of environmental surroundings on personal well-being in urban China using a multi-item well-being indicator

We examine the relationship between atmospheric and water pollution, traffic congestion, access to parkland and personal well-being using a survey administered across six Chinese cities in 2007. In contrast to existing studies of well-being determinants by economists which typically employ single-item indicators, we use the Personal Well-being Index (PWI). We also employ the Job Satisfaction Survey (JSS) to measure job satisfaction, which is one of the variables for which we control when examining the relationship between environmental surroundings and personal well-being. Previous research by psychologists has shown the PWI and JSS to have good psychometric properties in western and Chinese samples. A robust finding is that in cities with higher levels of atmospheric pollution and traffic congestion, respondents report lower levels of personal well-being ceteris paribus. Specifically, we find that a one standard deviation increase in suspended particles or sulphur dioxide emissions is roughly equivalent to a 12-13% reduction in average monthly income in the six cities. © 2010 Springer Science+Business Media, LLC.

Environmental invitingness for transport-related cycling in middle-aged adults: a proof of concept study using photographs

Introduction: Current evidence on associations between modifiable environmental characteristics and transport-related cycling remains inconsistent. Most studies on these associations used questionnaires to determine environmental perceptions, but such tools may be subject to bias due to unreliable recall. Moreover, questionnaires only measure separate environmental characteristics, while real environments are a combination of different characteristics. To overcome these limitations, the present proof of concept study used panoramic photographs of cycling environments to capture direct responses to the physical environment. We examined which depicted environmental characteristics were associated to environments' invitingness for transportation cycling. Furthermore, interactions with gender and participants' cycling behavior were examined. Methods: Fifty-nine middle-aged adults were recruited through purposeful convenience sampling. During a home visit, participants took part in a structured interview assessing demographics and PA during the preceding seven days, followed by an intuitive choice task and a (cognitive) rating task, which both measured 40 photographed environments' invitingness to cycle along. Multi-level cross-classified analyses were conducted using MLwiN 2.26. Results: Both tasks' multivariate results showed that presence of vegetation was identified as the most important environmental characteristic to invite people for engaging in transportation cycling, even when the amount of vegetation was relatively small. In the bivariate analyzes, some differences between results of the cognitive rating task and the intuitive choice task were found, showing that invitingness measured by the rating task was associated with environmental maintenance and cycling infrastructure, whereas invitingness determined by the choice task was associated with more traffic-oriented characteristics. Moreover, only for the choice task's results, moderating effects of gender and participants' cycling behavior in the preceding week were observed. Conclusion: The present study provides proof of concept that capturing people's less cognitive, more intuitive responses to an environment's invitingness for transport-related cycling may be important for revealing environment-behavior associations. If replicated in future studies using larger samples, results of our innovative measurements with photographs, especially those on vegetation, can complete the existing knowledge on which environmental characteristics are important for transportation cycling in adults and could form a basis to inform health promoters and local policy makers. However, future studies replicating our study method in larger samples and other population subgroups are highly encouraged. Moreover, causal relationships should be explored.

Environmental and genetic determinants of vitamin D insufficiency in 12-month-old infants

We aimed to investigate the relationship between genetic and environmental exposure and vitamin D status at age one, stratified by ethnicity. This study included 563 12-month-old infants in the HealthNuts population-based study. DNA from participants' blood samples was genotyped using Sequenom MassARRAY MALDI-TOF system on 28 single nucleotide polymorphisms (SNPs) in six genes. Using logistic regression, we examined associations between environmental exposure and SNPs in vitamin D pathway and filaggrin genes and vitamin D insufficiency (VDI). VDI, defined as serum 25-hydroxyvitamin D3(25(OH)D3) level ≤50 nmol/L, was measured using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Infants were stratified by ethnicity determined by parent's country of birth. Infants formula fed at 12 months were associated with reduced odds of VDI compared to infants with no current formula use at 12 months. This association differed by ethnicity (P;bsubesub;= 0.01). The odds ratio (OR) of VDI was 0.29 for Caucasian infants (95% CI, 0.18-0.47) and 0.04 for Asian infants (95% CI, 0.006-0.23). Maternal vitamin D supplementation during pregnancy and/or breastfeeding were associated with increased odds of infants being VDI (OR, 2.39; 95% CI, 1.11-5.18 and OR, 2.5; 95% CI, 1.20-5.24 respectively). Presence of a minor allele for any GC SNP (rs17467825, rs1155563, rs2282679, rs3755967, rs4588, rs7041) was associated with increased odds of VDI. Caucasian infants homozygous (AA) for rs4588 had an OR of 2.49 of being associated with VDI (95% CI, 1.19-5.18). In a country without routine infant vitamin D supplementation or food chain fortification, formula use is strongly associated with a reduced risk of VDI regardless of ethnicity. There was borderline significance for an association between filaggrin mutations and VDI. However, polymorphisms in vitamin D pathway related genes were associated with increased likelihood of being VDI in infancy. © 2014 Elsevier B.V. All rights reserved.

Integrated Sr isotope variations and global environmental changes through the late permian to early late triassic

New ⁸⁷Sr/⁸⁶Sr data based on 127 well-preserved and well-dated conodont samples from South China were measured using a new technique (LA-MC-ICPMS) based on single conodont albid crown analysis. These reveal a spectacular climb in seawater ⁸⁷Sr/⁸⁶Sr ratios during the Early Triassic that was the most rapid of the Phanerozoic. The rapid increase began in Bed 25 of the Meishan section (GSSP of the Permian-Triassic boundary, PTB), and coincided closely with the latest Permian extinction. Modeling results indicate that the accelerated rise of ⁸⁷Sr/⁸⁶Sr ratios can be ascribed to a rapid increase (>2.8×) of riverine flux of Sr caused by intensified weathering. This phenomenon could in turn be related to an intensification of warming-driven runoff and vegetation die-off. Continued rise of ⁸⁷Sr/⁸⁶Sr ratios in the Early Triassic indicates that continental weathering rates were enhanced >1.9 times compared to those of the Late Permian. Continental weathering rates began to decline in the middle-late Spathian, which may have played a role in the decrease of oceanic anoxia and recovery of marine benthos. The ⁸⁷Sr/⁸⁶Sr values decline gradually into the Middle Triassic to an equilibrium values around 1.2 times those of the Late Permian level, suggesting that vegetation coverage did not attain pre-extinction levels thereby allowing higher runoff.

Environmental barriers and enablers to physical activity participation among rural adults: A qualitative study

Issue addressed Social-ecological models of health behaviour acknowledge environmental influences, but research examining how the environment shapes physical activity in rural settings is limited. This study aimed to explore the environmental factors that act as barriers or facilitators to physical activity participation among rural adults. Methods Forty-nine adults from three regions of rural Tasmania, Australia, participated in semi-structured interviews that explored features of the environment that supported or hindered physical activity. Interviews were digitally recorded, transcribed verbatim and analysed thematically. Results Four key themes emerged: functionality, diversity, spaces and places for all and realistic expectations. 'Functionality' included connectivity with other destinations, distance, safety, continuity, supporting infrastructure and surfacing. While there was limited 'diversity' of structured activities and recreational facilities, the importance of easy and convenient access to a natural environment that accommodated physical activity was highlighted. 'Spaces and places for all' highlighted the importance of shared-use areas, particularly those that were family-and dog-friendly. Despite desires for more physical activity opportunities, many participants had 'realistic expectations' of what was feasible in rural settings. Conclusions Functionality, diversity, spaces and places for all and realistic expectations were identified as considerations important for physical activity among rural adults. Further research using quantitative approaches in larger samples is needed to confirm these findings. So what? Urban-centric views of environmental influences on physical activity are unlikely to be entirely appropriate for rural areas. Evidence-based recommendations are provided for creating new or modifying existing infrastructure to support active living in rural settings.